Studies on microbial glycerol dehydrogenase. Part III. Further characterization of glycerol dehydrogenase from Cellulomonas sp. NT3060.
نویسندگان
چکیده
منابع مشابه
Structure of glycerol dehydrogenase from Serratia.
The 1.90 Å resolution X-ray crystal structure of glycerol dehydrogenase derived from contaminating bacteria present during routine Escherichia coli protein expression is presented. This off-target enzyme showed intrinsic affinity for Ni(2+)-Sepharose, migrated at the expected molecular mass for the target protein during gel filtration and was crystallized before it was realised that contaminati...
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In the course of studies of t.he reduction of Furacin (5-nitro-2-furaldehyde semicarbazone) by cell-free extracts of a strain of Escherichia co& the presence of a number of diphosphopyridine nucleotide-linked dehydrogenases was indicated by the ability of such extract’s when dialyzed to reduce the nitrofuran only on the addition of diphosphopyridine nucleotide (DPN) and any one of a number of o...
متن کاملAnaerobic Glycerol-3-Phosphate Dehydrogenase and the glpD Operon Encoding Aerobic Glycerol-3-Phosphate Dehydrogenase in Escherichia coli: Further Characterization of Respiratory Control
In Escherichia coli, sn-glycerol-3-phosphate can be oxidized by two different flavo-dehydrogenases, an anaerobic enzyme encoded by the glpACB operon and an aerobic enzyme encoded by the glpD operon. These two operons belong to the glp regulon specifying the utilization of glycerol, sn-glycerol-3-phosphate, and glycerophosphodiesters. In glpR mutant cells grown under conditions of low catabolite...
متن کاملGlycerol dehydrogenase. structure, specificity, and mechanism of a family III polyol dehydrogenase.
BACKGROUND Bacillus stearothermophilus glycerol dehydrogenase (GlyDH) (glycerol:NAD(+) 2-oxidoreductase, EC 1.1.1.6) catalyzes the oxidation of glycerol to dihydroxyacetone (1,3-dihydroxypropanone) with concomitant reduction of NAD(+) to NADH. Analysis of the sequence of this enzyme indicates that it is a member of the so-called iron-containing alcohol dehydrogenase family. Despite this sequenc...
متن کاملGlycerol-3-phosphate dehydrogenase homologue from Schizosaccharomyces pombe.
A gene encoding an S.pombe homologue of glycerol-3-phosphate dehydrogenase was isolated from a XEMBL3 library. The clone was originally isolated because it hybridised to a rat BiP cDNA probe (1). Upon sequencing, however, it was found that there was no homology between this clone and BiP at the amino acid level. The open reading frame was used to search databases and the gene identified as an S...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1984
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.48.1603